OligoHydrogelArray (OHA) for Parallelized Solid‐Phase Extraction of Oligonucleotides

Immobilization of oligonucleotides on solid surfaces is an important step in many experimental workflows biology, such as gene expression analysis, genotyping, and drug discoveries. Capturing a highly efficient miniaturized format still challenging. In this work, preparation functionalized acrylamide hydrogels droplet microarray (DMA) chip reported. By further modification these through the Cu-catalyzed alkyne-azide cycloaddition, are attached covalently, creating OligoHydrogelArray (OHA). OHA enables hybridization with defined sequences parallelized, miniaturized, high-throughput manner overall loading capacity extraction efficiency suitable for most biological chemical applications. This system parallelized solid-phase target out mixture purification enrichment DNA/RNA/PNA application fields transcriptomics. current study, establish procedure reproducible determine key characteristics, height, diameter, shape gel pads well within material. Finally, ability to extract complementary oligonucleotide strands solution demonstrated set course future